FrlB

• Description: fructoselysine-6-P-glycosidase
  
  

Gene name	frlB
Synonyms	yurP
Essential	no
Product	fructoselysine-6-P-glycosidase
Function	metabolism of aminoacylated fructose
Gene expression levels in SubtiExpress: frlB
Metabolic function and regulation of this protein in SubtiPathways: Alternative nitrogen sources
MW, pI	36 kDa, 5.442
Gene length, protein length	984 bp, 328 aa
Immediate neighbours	frlO, yurQ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, utilization of nitrogen sources other than amino acids, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CodY regulon, FrlR regulon

The gene

Basic information

• Locus tag: BSU32610

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-48 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane associated PubMed

Database entries

• Structure: 3EUA

• UniProt: O32157

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • frlB-frlO-frlN-frlM-frlD PubMed
  • frlB-frlO PubMed
  • frlB PubMed

• Expression browser: frlB PubMed

• Sigma factor:

• Regulation:
  • frlB: repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • frlB: induced in the presence of fructosamine (FrlR) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed1, PubMed2
  • FrlR: transcription repression PubMed

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • the frlB-frlO-frlN-frlM-frlD operon is not expressed in a cshA mutant PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947