cshA

cshA
168

DEAD-box RNA helicase, contributes to efficient 3' exonucleolytic mRNA decay (together with PNPase), important for adaptation to low temperatures

Locus
BSU_04580
Molecular weight
57.11 kDa
Isoelectric point
9.89
Protein length
511 aaSequenceBlast
Gene length
1536 bpSequenceBlast
Function
RNA helicase
Product
DEAD-box RNA helicase
Essential
no
Synonyms
cshA, ydbR
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0513 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
511,106 → 512,641
Phenotypes of a mutant
poor growth at low temperatures (16 to 20°C) PubMed
reduced number of ribosomes PubMed
no expression of the frlB-frlO-frlN-frlM-frlD operon PubMed
strongly increased expression of the pftA-pftB operon PubMed
transcription profile resulting from cshA deletion: GEO PubMed
The protein
Catalyzed reaction/ biological activity
RNA helicase
required for ribosome assembly (biogenesis of the large subunit) PubMed
unwind duplex RNA with or without overhangs PubMed
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Protein family
helicase family (according to UniProt)
DEAD-box RNA helicases (according to UniProt)
Domains
Helicase ATP-binding domain (aa 34-204) (according to UniProt)
Helicase C-terminal domain (aa 215-375) (according to UniProt)
Structure
5IVL (PDB) PubMed
P96614 (AlphaFold)
Paralogous protein(s)
DeaD, YfmL, CshB
Localization
cytoplasma, colocalizes with the ribosomes PubMed, may also associate with the cell membrane PubMed
Expression and Regulation
Operons
Genes
cshA
Description
PubMed
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cshA

2025-03-10 05:29:32

Jstuelk

95

2bdc6c6b3c0715f63ff8646018e858bf68d537d5

D92CC40F338929DBD2BA0C50D3462B7FF865BD88

Biological materials
Mutant
GP1035 (ΔcshA::aphA3), available in Jörg Stülke's lab PubMed
GP1083 (ΔcshA::cat), available in Jörg Stülke's lab
BKE04580 (ΔcshA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTATAAAACTGCCCTTT,  downstream forward: _UP4_TAATTTGATCGATTCAGAGC
BKK04580 (ΔcshA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTATAAAACTGCCCTTT,  downstream forward: _UP4_TAATTTGATCGATTCAGAGC
Expression vectors
for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1387, available in Jörg Stülke's lab
for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1026 (aphA3), available in Jörg Stülke's lab
for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP1386, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed
FLAG-tag construct
GP1010 (spc, based on pGP1331), available in Jörg Stülke's lab
GP1074 (tet), available in Jörg Stülke's lab
GFP fusion
pGP1369 for chromosomal expression of CshA-YFP, available in Jörg Stülke's lab
B. subtilis GP1081 cshA-gfp spc, available in Jörg Stülke's lab,
GP1721 (in pBP43), expression of  cshA-GFP::spc under the native promoter, available in Jörg Stülke's lab PubMed
Labs working on this gene/protein
Mohamed Marahiel, Marburg University, Germany homepage
References
Reviews
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Original Publications
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E169F9711635457EA65FA71CCE9D7FF29DD8DF02

Page visits: 7736

Time of last update: 2025-03-31 02:49:52

Author of last update: Jstuelk