MutS

• Description: DNA mismatch repair (mismatch recognition protein)
  
  

Gene name	mutS
Synonyms
Essential	no
Product	mismatch recognition protein
Function	DNA repair
Gene expression levels in SubtiExpress: mutS
Interactions involving this protein in SubtInteract: MutS
MW, pI	97 kDa, 5.189
Gene length, protein length	2574 bp, 858 aa
Immediate neighbours	cotE, mutL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU17040

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • MutS recognizes mismatches in the DNA and recruits MutL to the site of mismatch recognition

• Protein family: DNA mismatch repair mutS family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • MutS-DnaN PubMed
  • DnaE-MutS PubMed

• Localization:
  • forms foci at midcell position, the frequency of foci increases upon mismatch formation PubMed
  • replication-active region of the DNA, via DnaN PubMed

Database entries

• Structure:

• UniProt: P49849

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mutS-mutL PubMed

• Expression browser: mutS PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • An antisense RNA is predicted for mutS PubMed

Biological materials

• Mutant: GP1190 (del mutSL::aphA3) available in the Stülke lab
  • 1A833 ( mutS::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Justin S Lenhart, Anushi Sharma, Manju M Hingorani, Lyle A Simmons
DnaN clamp zones provide a platform for spatiotemporal coupling of mismatch detection to DNA replication.
Mol Microbiol: 2013, 87(3);553-68
[PubMed:23228104] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Nicole M Dupes, Brian W Walsh, Andrew D Klocko, Justin S Lenhart, Heather L Peterson, David A Gessert, Cassie E Pavlick, Lyle A Simmons
Mutations in the Bacillus subtilis beta clamp that separate its roles in DNA replication from mismatch repair.
J Bacteriol: 2010, 192(13);3452-63
[PubMed:20453097] [WorldCat.org] [DOI] (I p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Mario Pedraza-Reyes, Ronald E Yasbin
Contribution of the mismatch DNA repair system to the generation of stationary-phase-induced mutants of Bacillus subtilis.
J Bacteriol: 2004, 186(19);6485-91
[PubMed:15375129] [WorldCat.org] [DOI] (P p)

F Ginetti, M Perego, A M Albertini, A Galizzi
Bacillus subtilis mutS mutL operon: identification, nucleotide sequence and mutagenesis.
Microbiology (Reading): 1996, 142 ( Pt 8);2021-9
[PubMed:8760914] [WorldCat.org] [DOI] (P p)