RsiW

Gene name	rsiW
Synonyms	ybbM
Essential	no
Product	anti-SigW
Function	control of SigW activity
Gene expression levels in SubtiExpress: rsiW
Interactions involving this protein in SubtInteract: RsiW
MW, pI	23 kDa, 6.646
Gene length, protein length	624 bp, 208 aa
Immediate neighbours	sigW, ybbP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

AbrB regulon, SigW regulon

The gene

Basic information

• Locus tag: BSU01740

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: anti-sigma-W factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: degraded by RasP and PrsW under conditions of alkali shock or in the presence of antimicrobial peptides, respectively. This results in the release of SigW

• Interactions:
  • PrsW-RsiW (proteolysis) PubMed
  • RasP-RsiW (proteolysis), RsiW-SigW

• Localization: membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: Q45588

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sigW-rsiW PubMed

• Expression browser: rsiW PubMed

• Sigma factor: SigW PubMed

• Regulation:
  • induced under conditions of alkali shock or in the presence of the toxin SdpC (SigW) PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Thomas Wiegert, University of Bayreuth, Germany Homepage

References

Reviews

Additional reviews: PubMed

Gu Chen, Xu Zhang
New insights into S2P signaling cascades: regulation, variation, and conservation.
Protein Sci: 2010, 19(11);2015-30
[PubMed:20836086] [WorldCat.org] [DOI] (I p)