DctS

• Description: two-component sensor kinase, regulation of the dctS-dctR-dctP operon
  
  

Gene name	dctS
Synonyms	ydbF
Essential	no
Product	two-component sensor kinase
Function	regulation of the dctS-dctR-dctP operon
Interactions involving this protein in SubtInteract: DctS
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	59 kDa, 6.198
Gene length, protein length	1605 bp, 535 aa
Immediate neighbours	dctB, dctR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources, protein modification, transcription factors and their control, membrane proteins, phosphoproteins

The gene

Basic information

• Locus tag: BSU04450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of DctR

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DctS-DctR

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P96601

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dctS-dctR-dctP PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP225 (dctS::cat), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kei Asai, Sang-Hoon Baik, Yasuhiro Kasahara, Shigeki Moriya, Naotake Ogasawara
Regulation of the transport system for C4-dicarboxylic acids in Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 2);263-271
[PubMed:10708364] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)