CshB

• Description: DEAD-box RNA helicase
  
  

Gene name	cshB
Synonyms	yqfR
Essential	no
Product	DEAD-box RNA helicase
Function	RNA helicase
MW, pI	49 kDa, 9.89
Gene length, protein length	1314 bp, 438 aa
Immediate neighbours	nfo, yqfQ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU25140

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: RNA helicase

• Protein family: DEAD box helicase family (according to Swiss-Prot) DEAD-box RNA helicase

• Paralogous protein(s): CshA, DeaD, YfmL

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: CshB-CspB PubMed, CshB-DeaD PubMed

• Localization: cytoplasma, colocalizes with the ribosomes PubMed

Database entries

• Structure:

• UniProt: P54475

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • well expressed in complex medium, poorly expressed in minimal medium PubMed
  • expression declines in stationary phase PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1002 (erm), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• FLAG-tag construct: GP1011 (spc, based on pGP1331), available in the Stülke labs

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

References

Franck Pandiani, Julien Brillard, Isabelle Bornard, Caroline Michaud, Stéphanie Chamot, Christophe Nguyen-the, Véronique Broussolle
Differential involvement of the five RNA helicases in adaptation of Bacillus cereus ATCC 14579 to low growth temperatures.
Appl Environ Microbiol: 2010, 76(19);6692-7
[PubMed:20709848] [WorldCat.org] [DOI] (I p)

Martin Lehnik-Habrink, Henrike Pförtner, Leonie Rempeters, Nico Pietack, Christina Herzberg, Jörg Stülke
The RNA degradosome in Bacillus subtilis: identification of CshA as the major RNA helicase in the multiprotein complex.
Mol Microbiol: 2010, 77(4);958-71
[PubMed:20572937] [WorldCat.org] [DOI] (I p)

Karen Hunger, Carsten L Beckering, Frank Wiegeshoff, Peter L Graumann, Mohamed A Marahiel
Cold-induced putative DEAD box RNA helicases CshA and CshB are essential for cold adaptation and interact with cold shock protein B in Bacillus subtilis.
J Bacteriol: 2006, 188(1);240-8
[PubMed:16352840] [WorldCat.org] [DOI] (P p)

Carsten L Beckering, Leif Steil, Michael H W Weber, Uwe Völker, Mohamed A Marahiel
Genomewide transcriptional analysis of the cold shock response in Bacillus subtilis.
J Bacteriol: 2002, 184(22);6395-402
[PubMed:12399512] [WorldCat.org] [DOI] (P p)