BmrD

• Description: multidrug ABC transporter (ATP-binding protein), also involved in the signalling pathway to activate KinA at the onset of sporulation
  
  

Gene name	bmrD
Synonyms	yheH
Essential	no
Product	multidrug ABC transporter (ATP-binding protein)
Function	multiple antibiotic resistance
Gene expression levels in SubtiExpress: bmrD
Interactions involving this protein in SubtInteract: BmrD
MW, pI	76 kDa, 7.082
Gene length, protein length	2019 bp, 673 aa
Immediate neighbours	bmrC, yheG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, resistance against toxins/ antibiotics, membrane proteins, sporulation proteins

This gene is a member of the following regulons

AbrB regulon, BmrB regulon

The gene

Basic information

• Locus tag: BSU09720

Database entries

• BsubCyc: BSU09720

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: has both a membrane-spanning and an ATP-binding domain PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • BmrC-BmrD PubMed

• Localization: cell membrane PubMed

Database entries

• BsubCyc: BSU09720

• Structure:

• UniProt: O07549

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: bmrB-bmrC-bmrD PubMed

• Expression browser: bmrD PubMed

• Sigma factor:

• Regulation:
  • induced in response to antibiotics that target protein synthesis (BmrB) PubMed
  • bmrC-bmrD expression only occurs during the late-exponential and stationary growth stages, irrespective of the timing of the antibiotic challenge (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed
  • BmrB: transcriptional attenuation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ewoud Reilman, Ruben A T Mars, Jan Maarten van Dijl, Emma L Denham
The multidrug ABC transporter BmrC/BmrD of Bacillus subtilis is regulated via a ribosome-mediated transcriptional attenuation mechanism.
Nucleic Acids Res: 2014, 42(18);11393-407
[PubMed:25217586] [WorldCat.org] [DOI] (I p)

Carmen Galián, Florence Manon, Manuela Dezi, Cristina Torres, Christine Ebel, Daniel Lévy, Jean-Michel Jault
Optimized purification of a heterodimeric ABC transporter in a highly stable form amenable to 2-D crystallization.
PLoS One: 2011, 6(5);e19677
[PubMed:21602923] [WorldCat.org] [DOI] (I p)

Cristina Torres, Carmen Galián, Christoph Freiberg, Jean-Raphaël Fantino, Jean-Michel Jault
The YheI/YheH heterodimer from Bacillus subtilis is a multidrug ABC transporter.
Biochim Biophys Acta: 2009, 1788(3);615-22
[PubMed:19167342] [WorldCat.org] [DOI] (P p)

Sanae Fukushima, Mika Yoshimura, Taku Chibazakura, Tsutomu Sato, Hirofumi Yoshikawa
The putative ABC transporter YheH/YheI is involved in the signalling pathway that activates KinA during sporulation initiation.
FEMS Microbiol Lett: 2006, 256(1);90-7
[PubMed:16487324] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)