FlgL

• Description: flagellar hook-associated protein 3 (HAP3), thought to form the hook-filament junction upon which the FliD cap is assembled
  
  

Gene name	flgL
Synonyms	yviD
Essential	no
Product	flagellar hook-associated protein 3 (HAP3)
Function	motility and chemotaxis
Gene expression levels in SubtiExpress: flgL
MW, pI	32 kDa, 4.42
Gene length, protein length	894 bp, 298 aa
Immediate neighbours	yviE, flgK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

ComK regulon, DegU regulon, SigD regulon

The gene

Basic information

• Locus tag: BSU35400

Phenotypes of a mutant

• the mutation suppresses the mucoid phenotype of motA or motB mutants PubMed

Database entries

• BsubCyc: BSU35400

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial flagellin family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot)

Database entries

• BsubCyc: BSU35400

• Structure:

• UniProt: P96501

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • comFA-comFB-comFC-yvyF-flgM-flgN-flgK-flgL PubMed
  • yvyF-flgM-flgN-flgK-flgL PubMed
  • flgM-flgN-flgK-flgL PubMed

• Expression browser: flgL PubMed

• Sigma factor:
  • comFA: SigA PubMed
  • yvyF: SigD PubMed
  • flgM: SigD PubMed

• Regulation:

• Regulatory mechanism:
  • ComK: transcription activation PubMed
  • flgM: DegU-P: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jia Mun Chan, Sarah B Guttenplan, Daniel B Kearns
Defects in the flagellar motor increase synthesis of poly-γ-glutamate in Bacillus subtilis.
J Bacteriol: 2014, 196(4);740-53
[PubMed:24296669] [WorldCat.org] [DOI] (I p)

Yi-Huang Hsueh, Loralyn M Cozy, Lok-To Sham, Rebecca A Calvo, Alina D Gutu, Malcolm E Winkler, Daniel B Kearns
DegU-phosphate activates expression of the anti-sigma factor FlgM in Bacillus subtilis.
Mol Microbiol: 2011, 81(4);1092-108
[PubMed:21736639] [WorldCat.org] [DOI] (I p)

D B Mirel, P Lauer, M J Chamberlin
Identification of flagellar synthesis regulatory and structural genes in a sigma D-dependent operon of Bacillus subtilis.
J Bacteriol: 1994, 176(15);4492-500
[PubMed:8045879] [WorldCat.org] [DOI] (P p)

J A Londoño-Vallejo, D Dubnau
comF, a Bacillus subtilis late competence locus, encodes a protein similar to ATP-dependent RNA/DNA helicases.
Mol Microbiol: 1993, 9(1);119-31
[PubMed:8412657] [WorldCat.org] [DOI] (P p)