HypO

• Description: NAD(P)H-flavin nitroreductase
  
  

Gene name	hypO
Synonyms	yfkO
Essential	no
Product	NAD(P)H-flavin nitroreductase
Function	protection against NaOCl stress
Gene expression levels in SubtiExpress: hypO
MW, pI	25 kDa, 5.667
Gene length, protein length	663 bp, 221 aa
Immediate neighbours	treR, yfkN
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

HypR regulon

The gene

Basic information

• Locus tag: BSU07830

Phenotypes of a mutant

• a hypO ohrA mutant is more sensitive to NaOCl stress than ohrA single mutant PubMed

Database entries

• BsubCyc: BSU07830

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nitroreductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU07830

• Structure:

• UniProt: O34475

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hypO PubMed

• Expression browser: hypO PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced under disulfide stress conditions (diamide, NaOCl) (HypR) PubMed

• Regulatory mechanism:
  • HypR:transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 292 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 638 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Haike Antelmann, University of Greifswald, Germany

References

Original articles

Palm GJ, Khanh Chi B, Waack P, Gronau K, Becher D, Albrecht D, Hinrichs W, Read RJ, Antelmann H.
Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR.
Nucleic Acids Res. 2012, Jan 11. [Epub ahead of print]
PubMed