GerD

• Description: scaffold of the germinosome, required for clustering of germinant receptors in the spore inner membrane
  
  

Gene name	gerD
Synonyms
Essential	no
Product	lipoprotein
Function	germination
Gene expression levels in SubtiExpress: gerD
Interactions involving this protein in SubtInteract: GerD
MW, pI	20 kDa, 4.862
Gene length, protein length	555 bp, 185 aa
Immediate neighbours	salA, kbaA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigF regulon, SigG regulon

The gene

Basic information

• Locus tag: BSU01550

Phenotypes of a mutant

• defective in germination in response to nutrients PubMed

Database entries

• BsubCyc: BSU01550

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: required for clustering of germinant receptors in the spore inner membrane PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • has an N-terminal signal sequence and a signal for fixation to lipid PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • forms trimeric rope-like structures PubMed
  • GerD is part of the germinosome in the spore inner membrane PubMed
  • GerD-Lgt to fix GerD to diacylglycerol in the membrane PubMed

• Localization:
  • outer surface of the inner spore membrane PubMed
  • inner spore membrane, spore coat, cortex, germ cell wall, some GerD is also present in the soluble fraction PubMed

Database entries

• BsubCyc: BSU01550

• Structure: 4O8W PubMed

• UniProt: P16450

• KEGG entry: [3]

• E.C. number:

Additional information

• 3,500 molecules are present per spore PubMed

Expression and regulation

• Operon: gerD PubMed

• Expression browser: gerD PubMed

• Sigma factor: SigF PubMed, SigG PubMed

• Regulation:
  • expression level depends on sporulation conditions (medim composition, temperature) PubMed

• Regulatory mechanism:

• Additional information:
  • 3,500 molecules are present per spore PubMed

Biological materials

• Mutant:
  • 1A677 ( gerD::erm), PubMed, available at BGSC
  • 1G17 ( gerD::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Anne Moir lab

Labs working on this gene/protein

Anne Moir, University of Sheffield, UK