ExoA

• Description: apurinic/apyrimidinic endonuclease, multifunctional DNA-repair enzyme, important for spore dormance
  
  

Gene name	exoA
Synonyms
Essential	no
Product	apurinic/apyrimidinic endonuclease
Function	repair of oxidative DNA damage in spores
Gene expression levels in SubtiExpress: exoA
MW, pI	29 kDa, 4.592
Gene length, protein length	756 bp, 252 aa
Immediate neighbours	ccpB, rpsR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU40880

Phenotypes of a mutant

• an exoA nfo double mutant is impaired in germination and spore outgrowth due to the accumulation of DNA lesions, this can be rescued by inactivation of disA PubMed
• an exoA nfo double mutant is sensitive to radiation PubMed

Database entries

• BsubCyc: BSU40880

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• ExoA is functionally redundant with Nfo

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates (according to Swiss-Prot)

• Protein family: DNA repair enzymes AP/exoA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU40880

• Structure:

• UniProt: P37454

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: exoA-ccpB (according to DBTBS)

• Expression browser: exoA PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP898 (exoA::kan), available in Jörg Stülke's lab
  • GP1503 (exoA::kan, nfo::cat), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)