LiaS

• Description: two-component sensor kinase/phosphatase, response to bacitracin
  
  

Gene name	liaS
Synonyms	yvqE
Essential	no
Product	two-component sensor kinase
Function	control of LiaR activity in response to bacitracin
Gene expression levels in SubtiExpress: liaS
Interactions involving this protein in SubtInteract: LiaS
MW, pI	40 kDa, 7.333
Gene length, protein length	1080 bp, 360 aa
Immediate neighbours	liaR, liaF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, resistance against oxidative and electrophile stress, resistance against toxins/ antibiotics, membrane proteins, phosphoproteins

This gene is a member of the following regulons

LiaR regulon

The gene

Basic information

• Locus tag: BSU33090

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • autophosphorylation, phosphorylation of LiaR
  • dephosphorylation of LiaR in the absence of the stress signal PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:
  • LiaS kinase activity is inhibited by LiaF, phosphatase acitivity is maintained by LiaF in the absence of the stress signal PubMed

• Interactions:
  • LiaS-LiaR

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O32198

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • liaI-liaH-liaG-liaF-liaS-liaR PubMed
  • liaG-liaF-liaS-liaR PubMed

• Expression browser: liaS PubMed

• Sigma factor:
  • liaI: SigA PubMed
  • liaG: SigA PubMed

• Regulation:
  • liaI: induced by bacitracin and rhamnolipids (LiaR) PubMed
  • liaI: repressed during logarithmic growth (AbrB) PubMed, indirect regulation by AbrB PubMed
  • liaG: constitutive

• Regulatory mechanism:
  • LiaR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

References

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