RocE

• Description: amino acid permease
  
  

Gene name	rocE
Synonyms
Essential	no
Product	amino acid permease
Function	arginine, ornithine and citrulline uptake
Gene expression levels in SubtiExpress: rocE
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	51 kDa, 8.528
Gene length, protein length	1401 bp, 467 aa
Immediate neighbours	rocF, rocD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, utilization of amino acids, membrane proteins

This gene is a member of the following regulons

AhrC regulon, CodY regulon, RocR regulon, SigL regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU40330

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:
  • secondary amino acid transporter
  • amino acid, polyamine, organocation (APC) family of transporters, TC 2.A.3

• Paralogous protein(s): AapA, GabP, HutM, RocC, YbgF, YbxG, YdgF, YtnA, YvbW

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot), membrane associated PubMed

Database entries

• Structure:

• UniProt: P39137

• KEGG entry: [3]

• T.C. number: 2.A.3.1.11

Expression and regulation

• Operon: rocD-rocE-rocF PubMed

• Expression browser: rocE PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • induced by arginine (RocR, AhrC) PubMed
  • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • RocR, AhrC: transcription activation PubMed
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: