GlpF

• Description: glycerol facilitator
  
  

Gene name	glpF
Synonyms
Essential	no
Product	glycerol facilitator
Function	glycerol uptake
Gene expression levels in SubtiExpress: glpF
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	28 kDa, 9.506
Gene length, protein length	822 bp, 274 aa
Immediate neighbours	glpP, glpK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

AbrB regulon, CcpA regulon, GlpP regulon

The gene

Basic information

• Locus tag: BSU09280

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: View classification (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P18156

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: glpF-glpK PubMed

• Expression browser: glpF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (8-fold) (CcpA) PubMed
  • induction by glycerol (GlpP) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • GlpP-dependent RNA switch (transcriptional antitermination) PubMed
  • AbrB: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: