Phy

• Description: phytase
  
  

Gene name	phy
Synonyms	yodV, yzxA
Essential	no
Product	3-phytase
Function	untilization of inositol hexakisphosphate (phytate)
Gene expression levels in SubtiExpress: phy
MW, pI	41 kDa, 4.936
Gene length, protein length	1146 bp, 382 aa
Immediate neighbours	cgeB, yodU
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphate metabolism

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

• Locus tag: BSU19800

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Myo-inositol hexakisphosphate + H₂O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate (according to Swiss-Prot)

• Protein family: msrA Met sulfoxide reductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed

Database entries

• Structure: 3AMR

• UniProt: P42094

• KEGG entry: [3]

• E.C. number: 3.1.3.8

Expression and regulation

• Operon: phy PubMed

• Expression browser: phy PubMed

• Sigma factor: none PubMed

• Regulation:

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:
  • the gene is not expressed in B. subtilis due to the absene of a functional promoter PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Martha Guerrero-Olazarán, Lilí Rodríguez-Blanco, J Gerardo Carreon-Treviño, Juan A Gallegos-López, José M Viader-Salvadó
Expression of a Bacillus phytase C gene in Pichia pastoris and properties of the recombinant enzyme.
Appl Environ Microbiol: 2010, 76(16);5601-8
[PubMed:20601512] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Oliwia Makarewicz, Sarah Dubrac, Tarek Msadek, Rainer Borriss
Dual role of the PhoP approximately P response regulator: Bacillus amyloliquefaciens FZB45 phytase gene transcription is directed by positive and negative interactions with the phyC promoter.
J Bacteriol: 2006, 188(19);6953-65
[PubMed:16980498] [WorldCat.org] [DOI] (P p)

N C Ha, B C Oh, S Shin, H J Kim, T K Oh, Y O Kim, K Y Choi, B H Oh
Crystal structures of a novel, thermostable phytase in partially and fully calcium-loaded states.
Nat Struct Biol: 2000, 7(2);147-53
[PubMed:10655618] [WorldCat.org] [DOI] (P p)

S Roels, R Losick
Adjacent and divergently oriented operons under the control of the sporulation regulatory protein GerE in Bacillus subtilis.
J Bacteriol: 1995, 177(21);6263-75
[PubMed:7592393] [WorldCat.org] [DOI] (P p)