YtsJ

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  • Description: malic enzyme, forms a transhydrogenation cycle with MalS for balancing of NADPH

Gene name ytsJ
Synonyms
Essential no
Product NADP-dependent malate dehydrogenase
Function malate utilization
Gene expression levels in SubtiExpress: ytsJ
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 43 kDa, 5.046
Gene length, protein length 1230 bp, 410 aa
Immediate neighbours accD, dnaE
Sequences Protein DNA Advanced_DNA
Genetic context
YtsJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YtsJ expression.png















Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU29220

Phenotypes of a mutant

Poor growth with malate as single carbon source PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-malate + NADP+ = pyruvate + CO2 + NADPH (according to Swiss-Prot) malate <--> pyruvate
  • Protein family: malic enzymes family (according to Swiss-Prot)
  • Paralogous protein(s): MleA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: Cytoplasm (Homogeneous) PubMed cytoplasm protein PubMed

Database entries

  • Structure: 1WW8 (from Pyrococcus horikoshii, 46% identity, 63% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion: GP1432 (spc, based on pGP1870), available in the Stülke lab
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Your additional remarks

References

Additional publications: PubMed