McsA

• Description: modulator of CtsR-dependent repression, McsA activates kinase activity of McsB
  
  

Gene name	mcsA
Synonyms	yacH
Essential	no
Product	activator of McsB kinase activity
Function	control of CtsR activity
Interactions involving this protein in SubtInteract: McsA
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	20 kDa, 6.624
Gene length, protein length	555 bp, 185 aa
Immediate neighbours	ctsR, mcsB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, sporulation proteins, general stress proteins (controlled by SigB), heat shock proteins, phosphoproteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon, SigF regulon

The gene

Basic information

• Locus tag: BSU00840

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-169 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • McsA-McsB-CtsR, McsA-McsB

• Localization:

Database entries

• Structure:

• UniProt: P37569

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ctsR-mcsA-mcsB-clpC-radA-disA PubMed

• Expression browser: mcsA PubMed

• Sigma factor: SigA PubMed, SigB PubMed1 PubMed2, SigF PubMed

• Regulation:
  • expressed early during sporulation in the forespore (SigF) PubMed
  • induced by stress (SigB) PubMed
  • induced by heat (CtsR) PubMed

• Regulatory mechanism:
  • CtsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: mcsA::aphA3 available from the Gerth lab

• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Gerth lab

References

Additional publications: PubMed