MutL

• Description: DNA mismatch repair
  
  

Gene name	mutL
Synonyms
Essential	no
Product	DNA mismatch repair
Function	DNA repair
Interactions involving this protein in SubtInteract: MutL
MW, pI	70 kDa, 5.583
Gene length, protein length	1881 bp, 627 aa
Immediate neighbours	mutS, ymzD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU17050

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: DNA mismatch repair mutL/hexB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • MutL (C-terminal domain)-DnaN PubMed
  • DnaE-MutL PubMed

• Localization:
  • forms foci at midcell position, the frequency of foci increases upon mismatch formation PubMed

Database entries

• Structure: 3KDK (C-terminal domain) PubMed

• UniProt: P49850

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mutS-mutL PubMed

• Expression browser: mutL PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • A ncRNA is predicted between mutL and ymzD PubMed

Biological materials

• Mutant: GP1190 (del mutSL::aphA3) available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Nicholas J Bolz, Justin S Lenhart, Steven C Weindorf, Lyle A Simmons
Residues in the N-terminal domain of MutL required for mismatch repair in Bacillus subtilis.
J Bacteriol: 2012, 194(19);5361-7
[PubMed:22843852] [WorldCat.org] [DOI] (I p)

Monica C Pillon, Jessica J Lorenowicz, Michael Uckelmann, Andrew D Klocko, Ryan R Mitchell, Yu Seon Chung, Paul Modrich, Graham C Walker, Lyle A Simmons, Peter Friedhoff, Alba Guarné
Structure of the endonuclease domain of MutL: unlicensed to cut.
Mol Cell: 2010, 39(1);145-51
[PubMed:20603082] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Mario Pedraza-Reyes, Ronald E Yasbin
Contribution of the mismatch DNA repair system to the generation of stationary-phase-induced mutants of Bacillus subtilis.
J Bacteriol: 2004, 186(19);6485-91
[PubMed:15375129] [WorldCat.org] [DOI] (P p)

F Ginetti, M Perego, A M Albertini, A Galizzi
Bacillus subtilis mutS mutL operon: identification, nucleotide sequence and mutagenesis.
Microbiology (Reading): 1996, 142 ( Pt 8);2021-9
[PubMed:8760914] [WorldCat.org] [DOI] (P p)