ScoC

• Description: transcriptional repressor of genes expressed in the transition phase
  
  

Gene name	scoC
Synonyms	hpr, catA
Essential	no
Product	transcriptional repressor (MarR family)
Function	transition state regulator
Interactions involving this protein in SubtInteract: ScoC
Regulation of this protein in SubtiPathways: Biofilm, Alternative nitrogen sources
MW, pI	23 kDa, 5.188
Gene length, protein length	609 bp, 203 aa
Immediate neighbours	yhaI, yhaH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, transition state regulators

This gene is a member of the following regulons

AbrB regulon, SalA regulon, SenS regulon

The gene

Basic information

• Locus tag: BSU09990

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SinR-ScoC

• Localization:

Database entries

• Structure: 2FXA

• UniProt: P11065

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: scoC PubMed

• Expression browser: scoC PubMed

• Sigma factor:

• Regulation:
  • expressed upon transition into the stationary phase (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription activation PubMed
  • SenS: positive regulation PubMed
  • SalA: negative regulation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Bindiya Kaushal, Salbi Paul, F Marion Hulett
Direct regulation of Bacillus subtilis phoPR transcription by transition state regulator ScoC.
J Bacteriol: 2010, 192(12);3103-13
[PubMed:20382764] [WorldCat.org] [DOI] (I p)

Prashant Kodgire, K Krishnamurthy Rao
A dual mode of regulation of flgM by ScoC in Bacillus subtilis.
Can J Microbiol: 2009, 55(8);983-9
[PubMed:19898538] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Guojun Wang, Kozo Ochi
ScoC regulates bacilysin production at the transcription level in Bacillus subtilis.
J Bacteriol: 2009, 191(23);7367-71
[PubMed:19801406] [WorldCat.org] [DOI] (I p)

Prashant Kodgire, K Krishnamurthy Rao
hag expression in Bacillus subtilis is both negatively and positively regulated by ScoC.
Microbiology (Reading): 2009, 155(Pt 1);142-149
[PubMed:19118355] [WorldCat.org] [DOI] (P p)

Prashant Kodgire, Madhulika Dixit, K Krishnamurthy Rao
ScoC and SinR negatively regulate epr by corepression in Bacillus subtilis.
J Bacteriol: 2006, 188(17);6425-8
[PubMed:16923912] [WorldCat.org] [DOI] (P p)

Eiji Kawachi, Sadanobu Abe, Teruo Tanaka
Inhibition of Bacillus subtilis scoC expression by multicopy senS.
J Bacteriol: 2005, 187(24);8526-30
[PubMed:16321961] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Atsushi Matsuzawa, Hirofumi Yoshikawa, Teruo Tanaka
Bacillus subtilis SalA (YbaL) negatively regulates expression of scoC, which encodes the repressor for the alkaline exoprotease gene, aprE.
J Bacteriol: 2004, 186(10);3056-64
[PubMed:15126467] [WorldCat.org] [DOI] (P p)

Alejandro Sánchez, Jorge Olmos
Bacillus subtilis transcriptional regulators interaction.
Biotechnol Lett: 2004, 26(5);403-7
[PubMed:15104138] [WorldCat.org] [DOI] (P p)

Sasha H Shafikhani, Esperanza Núñez, Terrance Leighton
ScoC mediates catabolite repression of sporulation in Bacillus subtilis.
Curr Microbiol: 2003, 47(4);327-36
[PubMed:14629015] [WorldCat.org] [DOI] (P p)

A Koide, M Perego, J A Hoch
ScoC regulates peptide transport and sporulation initiation in Bacillus subtilis.
J Bacteriol: 1999, 181(13);4114-7
[PubMed:10383984] [WorldCat.org] [DOI] (P p)

P T Kallio, J E Fagelson, J A Hoch, M A Strauch
The transition state regulator Hpr of Bacillus subtilis is a DNA-binding protein.
J Biol Chem: 1991, 266(20);13411-7
[PubMed:1906467] [WorldCat.org] (P p)

B C Dowds, J A Hoch
Regulation of the oxidative stress response by the hpr gene in Bacillus subtilis.
J Gen Microbiol: 1991, 137(5);1121-5
[PubMed:1907636] [WorldCat.org] [DOI] (P p)

M A Strauch, G B Spiegelman, M Perego, W C Johnson, D Burbulys, J A Hoch
The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein.
EMBO J: 1989, 8(5);1615-21
[PubMed:2504584] [WorldCat.org] [DOI] (P p)

M Perego, J A Hoch
Sequence analysis and regulation of the hpr locus, a regulatory gene for protease production and sporulation in Bacillus subtilis.
J Bacteriol: 1988, 170(6);2560-7
[PubMed:3131303] [WorldCat.org] [DOI] (P p)