PfkA

• Description: is the main controling enzyme of the glycolysis. Phosphorylates fructose 6-phosphate to fructose 1,3-bisphosphate
  
  

Gene name	pfkA
Synonyms	pfk
Essential	yes
Product	6-phosphofructokinase
Function	catabolic enzyme in glycolysis
MW, pI	34,1 kDa, 6.14
Gene length, protein length	957 bp, 319 amino acids
Immediate neighbours	accA, pyk
Gene sequence (+200bp)	Protein sequence
Genetic context

The gene

Basic information

• Coordinates: 2985630 - 2986586

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate

• Protein family: phosphofructokinase family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • 3 x nucleotide binding domain (ATP) (21–25), (154–158), (171–187)

• Modification:

• Cofactor(s): ATP

• Effectors of protein activity:

• Interactions: Pyk-PfkA

• Localization: cytoplasm PubMed

Database entries

• Structure: Geobacillus stearothermophilus NCBI, Mutan, complex with Fructose-6-phosphate Geobacillus stearothermophilus NCBI

• Swiss prot entry: [3]

• KEGG entry: [4]

• E.C. number: [5]

Expression and regulation

• Operon: pfkA pyk

• Sigma factor:

• Regulation: twofold induced by glucose

• Regulatory mechanism:

• Additional information:

References

1. Commichau, F. M., Rothe, F. M., Herzberg, C., Wagner, E., Hellwig, D., Lehnik-Habrink, M., Hammer, E., Völker, U. & Stülke, J. Novel activities of glycolytic enzymes in Bacillus subtilis: Interactions with essential proteins involved in mRNA processing. subm.
2. Ludwig, H., Homuth, G., Schmalisch, M., Dyka, F. M., Hecker, M., and Stülke, J. (2001) Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon. Mol Microbiol 41, 409-422.PubMed