accA

accA
168

acetyl-CoA carboxylase (alpha subunit)

Locus
BSU_29200
Molecular weight
36.18 kDa
Isoelectric point
6.09
Protein length
325 aaSequenceBlast
Gene length
978 bpSequenceBlast
Function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
Product
acetyl-CoA carboxylase (alpha subunit))
Essential
yes
E.C.
6.4.1.2
Synonyms
accA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0825 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,987,731  2,988,708
Phenotypes of a mutant
essential PubMed
specific point mutations affecting AccA facilitate the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate PubMed
Additional information
ponA ecsA, ponA sigI, and ponA rasP double mutants have a growth defect that can be suppressed by reduced fatty acid synthesis (mutations in accA, accB, accC, accD or downregulation of the FapR regulon due to specific mutations in fapR) PubMed
The protein
Catalyzed reaction/ biological activity
acetyl-CoA + N6-carboxybiotinyl-L-lysyl-[protein] --> malonyl-CoA + N6-biotinyl-L-lysyl-[protein] (according to UniProt)
Protein family
accA family (single member, according to UniProt)
Domains
CoA carboxyltransferase C-terminal domain (aa 38-292) (according to UniProt)
Structure
2F9I (PDB) (from Staphylococcus aureus; equivalent to AccD-AccA, 64% identity) PubMed
O34847 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot),  Membrane-proximal (Spotty) PubMed
Expression and Regulation
Operons
Genes
accD-accA
Description
PubMed
Regulation
expressed under conditions that trigger sporulation (Spo0A) PubMed
Regulatory mechanism
Spo0A: activation, PubMed, in spo0A regulon
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accD→accA

2025-03-16 17:46:58

ghost

148

b652df2692748c745aab0f8ceb0e50c1ffcb2a82

36A9759B4AA4F3ACBABBC425410BB00129DBEA9D

Biological materials
Expression vectors
pGP1021: purification from E. coli with an N-terminal His-tag, with TEV cleavage site, (in pETM-11), available in Jörg Stülke's lab
pGP1024: purification from E. coli with an N-terminal Strep-tag, with TEV cleavage site, (in pGP172), available in Jörg Stülke's lab
pGP1723: purification from B. subtilis with an C-terminal Strep-tag, for SPINE, (in pGP382), available in Jörg Stülke's lab
pGP3696: expression of accDA by pBQ200 in B. subtilis, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
GP1487 (spc, based on pGP1331), available in Jörg Stülke's lab
GFP fusion
GP1490 (spc, based on pBP43), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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22B3039DF4D106F121DBC765F49878C9B324FDB2

Page visits: 6385

Time of last update: 2025-03-31 16:59:32

Author of last update: Dennis.wicke