HypO

• Description: NAD(P)H-flavin nitroreductase
  
  

Gene name	yfkO (hypO)
Synonyms	hypO
Essential	no
Product	NAD(P)H-flavin nitroreductase
Function	probably involved in NaOCl and diamide detoxification
MW, pI	25 kDa, 5.667
Gene length, protein length	663 bp, 221 aa
Immediate neighbours	treR, yfkN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

electron transport/ other, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

HypR regulon

The gene

Basic information

• Locus tag: BSU07830

Phenotypes of a mutant

hypO ohrA mutant more sensitive to NaOCl stress than ohrA single mutant

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nitroreductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34475

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ykfO (hypO) (according to DBTBS)

• Sigma factor:

• Regulation: induced under disulfide stress conditions (diamide, NaOCl) (HypR) PubMed

• Regulatory mechanism:
  • HypR:transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Gottfried J Palm, Bui Khanh Chi, Paul Waack, Katrin Gronau, Dörte Becher, Dirk Albrecht, Winfried Hinrichs, Randy J Read, Haike Antelmann
Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR.
Nucleic Acids Res: 2012, 40(9);4178-92
[PubMed:22238377] [WorldCat.org] [DOI] (I p)

G A Prosser, A V Patterson, D F Ackerley
uvrB gene deletion enhances SOS chromotest sensitivity for nitroreductases that preferentially generate the 4-hydroxylamine metabolite of the anti-cancer prodrug CB1954.
J Biotechnol: 2010, 150(1);190-4
[PubMed:20727918] [WorldCat.org] [DOI] (I p)