T-box
The T-box element is an RNA switch that controls genes involved in amino acid metabolism.
In the absence of an amino acid, the uncharged tRNA binds the T-box region in the leader region of the mRNA of the controlled gene/ operon and thereby prevents the formation of a transcription terminator. The result is gene expression of the target gene in the absence of the specific amino acid.
Complete list of genes controlled by T-box elements
- Induction by alanine limitation
- Induction by serine limitation
Structure of a T-box ribowsitch
Melinda S Gerdeman, Tina M Henkin, Jennifer V Hines
Solution structure of the Bacillus subtilis T-box antiterminator RNA: seven nucleotide bulge characterized by stacking and flexibility.
J Mol Biol: 2003, 326(1);189-201
[PubMed:12547201]
[WorldCat.org]
[DOI]
(P p)
Reviews on T-box regulation
Nicholas J Green, Frank J Grundy, Tina M Henkin
The T box mechanism: tRNA as a regulatory molecule.
FEBS Lett: 2010, 584(2);318-24
[PubMed:19932103]
[WorldCat.org]
[DOI]
(I p)
Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532]
[WorldCat.org]
[DOI]
(I p)
T M Henkin, F J Grundy
Sensing metabolic signals with nascent RNA transcripts: the T box and S box riboswitches as paradigms.
Cold Spring Harb Symp Quant Biol: 2006, 71;231-7
[PubMed:17381302]
[WorldCat.org]
[DOI]
(P p)
Frank J Grundy, Tessa R Moir, Margaret T Haldeman, Tina M Henkin
Sequence requirements for terminators and antiterminators in the T box transcription antitermination system: disparity between conservation and functional requirements.
Nucleic Acids Res: 2002, 30(7);1646-55
[PubMed:11917026]
[WorldCat.org]
[DOI]
(I p)
M Pelchat, J Lapointe
Aminoacyl-tRNA synthetase genes of Bacillus subtilis: organization and regulation.
Biochem Cell Biol: 1999, 77(4);343-7
[PubMed:10546897]
[WorldCat.org]
(P p)
T M Henkin
tRNA-directed transcription antitermination.
Mol Microbiol: 1994, 13(3);381-7
[PubMed:7527891]
[WorldCat.org]
[DOI]
(P p)
F J Grundy, T M Henkin
tRNA as a positive regulator of transcription antitermination in B. subtilis.
Cell: 1993, 74(3);475-82
[PubMed:8348614]
[WorldCat.org]
[DOI]
(P p)
