tyrZ

tyrZ
168

alternative tyrosyl-tRNA synthetase, required to prevent misincorporation of D-Tyr into proteins

Locus
BSU_38460
Molecular weight
46.94 kDa
Isoelectric point
6.66
Protein length
413 aaSequenceBlast
Gene length
1242 bpSequenceBlast
Function
translation
Product
tyrosyl-tRNA synthetase (minor)
Essential
no
E.C.
6.1.1.1
Synonyms
tyrZ, ipa-9r, tyrS1, tyrT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0162 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,947,158  3,948,399
Phenotypes of a mutant
sensitive against D-Tyr PubMed
a ''tyrS tyrZ'' double mutant is not viable PubMed
The protein
Catalyzed reaction/ biological activity
ATP + L-tyrosine + tRNATyr --> AMP + diphosphate + H+ + L-tyrosyl-tRNATyr (according to UniProt)
TyrZ is highly specific for L-Tyr, prevents misincorporation of D-Tyr into proteins, less processive than TyrS PubMed
Protein family
S4 RNA-binding domain superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
Class-I aminoacyl-tRNA synthetase family (according to UniProt)
Domains
S4 RNA-binding domain (aa 353-413) (according to UniProt)
Structure
6BQY (PDB) (from Acinetobacter baumannii, 46% identity)
P25151 (AlphaFold)
Modification
phosphorylated on Thr-311 PubMed
Paralogous protein(s)
TyrS
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
dtrR-tyrZ
Description
PubMed
Regulation
induced by tyrosine limitation (T-box) PubMed
Regulatory mechanism
DtrR: repression, PubMed, in dtrR regulon
T-box: RNA switch, in T-box
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dtrR→tyrZ

2025-03-17 19:38:05

ghost

74

74b2f4d2d40be21d04649012c98c32ffad1c5306

AE76E703ABACEA6477FFDE49A728A198DC210002

Biological materials
Mutant
QB53532 (aphA3), available in Jörg Stülke's lab
BKE38460 (tyrZ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTCCTCCTTTTTTAC,  downstream forward: _UP4_TAATCTGATATTCCGAAACG
BKK38460 (tyrZ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTCCTCCTTTTTTAC,  downstream forward: _UP4_TAATCTGATATTCCGAAACG
References
Reviews
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Original Publications
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1968743631A20739B5DA8B489CB1E7E42F3B93E3

Page visits: 3148

Time of last update: 2025-03-31 19:23:57

Author of last update: Melvin.boenninger