HutP

• Description: transcriptional antiterminator of the hutH operon
  
  

Gene name	hutP
Synonyms	hutP1
Essential	no
Product	transcriptional antiterminator
Function	regulation of histidine utilization
Metabolic function and regulation of this protein in SubtiPathways: His
MW, pI	16 kDa, 6.057
Gene length, protein length	453 bp, 151 aa
Immediate neighbours	abn2, hutH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39340

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: hutP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3BOY (complex bound to the hut mRNA), 1WPT

• UniProt: P10943

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hutP-hutH-hutU-hutI-hutG-hutM PubMed

• Sigma factor: SigA

• Regulation: repressed by glucose (CcpA)
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism: CcpA: transcription repression
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Juliane Ollinger, Kyung-Bok Song, Haike Antelmann, Michael Hecker, John D Helmann
Role of the Fur regulon in iron transport in Bacillus subtilis.
J Bacteriol: 2006, 188(10);3664-73
[PubMed:16672620] [WorldCat.org] [DOI] (P p)

M Oda, N Kobayashi, Y Kurusu, M Fujita
Analysis of histidine-dependent antitermination in Bacillus subtilis hut operon.
Nucleic Acids Symp Ser: 2000, (44);5-6
[PubMed:12903241] [WorldCat.org] [DOI] (P p)

S H Fisher, K Rohrer, A E Ferson
Role of CodY in regulation of the Bacillus subtilis hut operon.
J Bacteriol: 1996, 178(13);3779-84
[PubMed:8682780] [WorldCat.org] [DOI] (P p)