abn2
168
endo-1,5-alpha-L-arabinosidase
Locus
BSU_39330
Molecular weight
52.38 kDa
Isoelectric point
7.37
Function
arabinan degradation
Product
endo-1,5-alpha-L-arabinosidase
Essential
no
E.C.
null
Synonyms
abn2, yxiA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3507 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,039,466 4,040,875
The protein
Catalyzed reaction/ biological activity
Endohydrolysis of (1->5)-alpha-arabinofuranosidic linkages in (1->5)-arabinans (according to UniProt)
Protein family
glycosyl hydrolase 43 family (according to UniProt)
N-terminal catalytic domain with a characteristic -propeller fold and a C-terminal domain whose function is unknown PubMed
Ca2+ PubMed
Structure
Kinetic information
With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50C. PubMed
extracellular (signal peptide) PubMed
Expression and Regulation
Operons
Genes
Description
Regulation
expression is stimulated by arabinose and pectin and repressed by glucose PubMed
Regulatory mechanism
Sigma factors
SigA: sigma factor, in sigA regulon
Biological materials
Mutant
MGNA-B716 (yxiA::erm), available at the NBRP B. subtilis, Japan
Labs working on this gene/protein
[http://www.itqb.unl.pt/research/biology/microbial-genetics [Isabel de Sa-Nogueira]], Lisboa, Portugal [homepage]
References
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Time of last update: 2025-03-29 13:00:42
Author of last update: Melvin.boenninger