RocR

From SubtiWiki
Revision as of 15:27, 20 July 2009 by Lflorez (talk | contribs)
Jump to: navigation, search
  • Description: transcriptional activator of arginine utilization operons

Gene name rocR
Synonyms
Essential no
Product transcriptional regulator
Function transcriptional activator of arginine utilization operons
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 52.6 kDa, 6.08
Gene length, protein length 1383 bp, 461 amino acids
Immediate neighbours rocD, yyxA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RocR context.gif
This image was kindly provided by SubtiList








The gene

Basic information

  • Locus tag: BSU40350

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription activation at SigL-dependent promoters of rocABC, rocDEF, and rocG
  • Protein family: Sigma-54 interacting transcription activator
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • Sigma-54 factor interaction domain (143–372)
    • 2x nucleotid-binding domain (171–178),(233–242)
    • HTH motif (434–453)
  • Modification:
  • Cofactor(s): ornithine or citrulline are required for RocR-dependent transcription activation PubMed
  • Effectors of protein activity:
  • Interactions: RocR-SigL
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon: rocR
  • Regulatory mechanism: autorepression
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

Your additional remarks

References