RocD

• Description: ornithine transaminase
  
  

Gene name	rocD
Synonyms
Essential	no
Product	ornithine transaminase
Function	arginine, ornithine and citrulline utilization
MW, pI	43 kDa, 4.894
Gene length, protein length	1203 bp, 401 aa
Immediate neighbours	rocE, rocR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU40340

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid (according to Swiss-Prot)

• Protein family: OAT subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P38021

• KEGG entry: [3]

• E.C. number: 2.6.1.13

Expression and regulation

• Operon: rocD-rocE-rocF PubMed

• Sigma factor: SigL PubMed

• Regulation: induced by arginine (RocR, AhrC) PubMed, repressed by CodY PubMed
  • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism: RocR, AhrC: transcription activation PubMed, CodY: transcription repression PubMed
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP656, aphA3, available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Gardan R, Rapoport G, Débarbouillé M. (1995) Expression of the rocDEF operon involved in arginine catabolism in Bacillus subtilis. J Mol Biol. Jun 23;249(5): 843-56. PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed