Difference between revisions of "YtsJ"
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === |
Revision as of 14:06, 2 June 2009
- Description: malic enzyme
Gene name | ytsJ |
Synonyms | |
Essential | no |
Product | NAD-dependent malate dehydrogenase |
Function | malate utilization |
MW, pI | 43 kDa, 5.046 |
Gene length, protein length | 1230 bp, 410 aa |
Immediate neighbours | accD, dnaE |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag:
Phenotypes of a mutant
Poor growth with malate as single carbon source PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-malate + NAD+ = pyruvate + CO2 + NADH (according to Swiss-Prot) malate <--> pyruvate
- Protein family: malic enzymes family (according to Swiss-Prot)
- Paralogous protein(s): MleA
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
Database entries
- Structure:
- Swiss prot entry: O34962
- KEGG entry: BSU29220
- E.C. number: 1.1.1.38 0
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP612 (spc), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Your additional remarks
References
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed