RecR

• Description: required for the formation of RecA DNA repair centers
  
  

Gene name	recR
Synonyms	recM
Essential	no
Product	formation of RecA DNA repair centers
Function	DNA repair/ recombination
Gene expression levels in SubtiExpress: recR
Interactions involving this protein in SubtInteract: RecR
MW, pI	21 kDa, 5.301
Gene length, protein length	594 bp, 198 aa
Immediate neighbours	yaaK, yaaL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU00210

Phenotypes of a mutant

• drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) PubMed

Database entries

• BsubCyc: BSU00210

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for the formation of RecA DNA repair centers (together with RecO) PubMed

• Protein family: recR family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RecR-RecO, RecR:RecO molecular ratio of 2:1 (as judged from other bacteria such as E. coli and Deinococcus radiodurans) PubMed

• Localization:
  • cytoplasm (homogeneous) PubMed

Database entries

• BsubCyc: BSU00210

• Structure:
  • 2V1C (the RecR-RecO complex from Deinococcus radiodurans, RecR: 52% identity, 78% similarity, RecO: 30%/ 58%) PubMed

• UniProt: P24277

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: recR PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

Justin S Lenhart, Eileen R Brandes, Jeremy W Schroeder, Roderick J Sorenson, Hollis D Showalter, Lyle A Simmons
RecO and RecR are necessary for RecA loading in response to DNA damage and replication fork stress.
J Bacteriol: 2014, 196(15);2851-60
[PubMed:24891441] [WorldCat.org] [DOI] (I p)

Ignacija Vlašić, Ramona Mertens, Elena M Seco, Begoña Carrasco, Silvia Ayora, Günther Reitz, Fabian M Commichau, Juan C Alonso, Ralf Moeller
Bacillus subtilis RecA and its accessory factors, RecF, RecO, RecR and RecX, are required for spore resistance to DNA double-strand break.
Nucleic Acids Res: 2014, 42(4);2295-307
[PubMed:24285298] [WorldCat.org] [DOI] (I p)

Joanna Timmins, Ingar Leiros, Sean McSweeney
Crystal structure and mutational study of RecOR provide insight into its mode of DNA binding.
EMBO J: 2007, 26(13);3260-71
[PubMed:17581636] [WorldCat.org] [DOI] (P p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

S Ayora, A C Stiege, R Lurz, J C Alonso
Bacillus subtilis 168 RecR protein-DNA complexes visualized as looped structures.
Mol Gen Genet: 1997, 254(1);54-62
[PubMed:9108290] [WorldCat.org] [DOI] (P p)