ComFA

• Description: membrane-associated ATPase, may provide energy for DNA transport
  
  

Gene name	comFA
Synonyms
Essential	no
Product	ATP-binding protein
Function	genetic competence
Gene expression levels in SubtiExpress: comFA
Interactions involving this protein in SubtInteract: ComFA
Metabolic function and regulation of this protein in SubtiPathways: ComFA
MW, pI	52 kDa, 9.824
Gene length, protein length	1389 bp, 463 aa
Immediate neighbours	comFB, yviA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, membrane proteins, phosphoproteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU35470

Phenotypes of a mutant

knockout has 1000-fold reduction in transformability compared to the wild-type PubMed

Database entries

• BsubCyc: BSU35470

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: helicase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-20, Arg-186, and Arg-446 PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • ComGA-ComFA PubMed
  • ComEC-ComFA PubMed
  • CoiA-ComFA PubMed
  • SsbB-ComFA PubMed
  • DprA-ComFA PubMed
  • RecA-ComFA PubMed

• Localization:
  • cell membrane PubMed
  • localizes to cell poles in competent cells PubMed
  • localizes to cell poles PubMed

Database entries

• BsubCyc: BSU35470

• Structure:

• UniProt: P39145

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: comFA-comFB-comFC-yvyF-flgM-flgN-flgK-flgL PubMed

• Expression browser: comFA PubMed

• Sigma factor: comFA: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat Rev Microbiol: 2004, 2(3);241-9
[PubMed:15083159] [WorldCat.org] [DOI] (P p)

Original publications

Additional publications: PubMed