Difference between revisions of "AccA"

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(Reviews)
(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
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** purification from ''B. subtilis'' with an C-terminal Strep-tag, for [[SPINE]], (in [[pGP382]]): pGP1723, available in [[Jörg Stülke]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
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* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:'''  
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* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
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 +
* '''FLAG-tag construct:'''
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** GP1487 (''spc'', based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 10:34, 6 March 2015

  • Description: acetyl-CoA carboxylase (alpha subunit)

Gene name accA
Synonyms
Essential yes PubMed
Product acetyl-CoA carboxylase (alpha subunit))
Function production of malonyl-CoA, the substrate for fatty acid biosynthesis
Gene expression levels in SubtiExpress: accA
Interactions involving this protein in SubtInteract: AccA
Metabolic function and regulation of this protein in SubtiPathways:
accA
MW, pI 36 kDa, 6.087
Gene length, protein length 975 bp, 325 aa
Immediate neighbours pfkA, accD
Sequences Protein DNA DNA_with_flanks
Genetic context
AccA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AccA expression.png















Categories containing this gene/protein

biosynthesis of lipids, essential genes

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

  • Locus tag: BSU29200

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA (according to Swiss-Prot)
  • Protein family: accA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2F9I (from Staphylococcus aureus; equivalent to AccD-AccA, 64% identity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1047 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 795 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 910 PubMed

Biological materials

  • Mutant:
  • Expression vector:
    • purification from B. subtilis with an C-terminal Strep-tag, for SPINE, (in pGP382): pGP1723, available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

L Tong
Acetyl-coenzyme A carboxylase: crucial metabolic enzyme and attractive target for drug discovery.
Cell Mol Life Sci: 2005, 62(16);1784-803
[PubMed:15968460] [WorldCat.org] [DOI] (P p)

Stephen W White, Jie Zheng, Yong-Mei Zhang, Rock
The structural biology of type II fatty acid biosynthesis.
Annu Rev Biochem: 2005, 74;791-831
[PubMed:15952903] [WorldCat.org] [DOI] (P p)

John E Cronan, Grover L Waldrop
Multi-subunit acetyl-CoA carboxylases.
Prog Lipid Res: 2002, 41(5);407-35
[PubMed:12121720] [WorldCat.org] [DOI] (P p)


Original Publications