Difference between revisions of "SkfA"
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* '''GFP fusion:''' | * '''GFP fusion:''' | ||
+ | ** GP1645 ''[[ymdB]]''::spc ''skf-gfp'' (spc) in the NCIB3610 background, available in [[ Jörg Stülke]]'s lab | ||
+ | |||
* '''two-hybrid system:''' | * '''two-hybrid system:''' |
Revision as of 15:51, 3 February 2015
- Description: spore killing factor
Gene name | skfA |
Synonyms | ybcO |
Essential | no |
Product | spore killing factor |
Function | killing of sister cells |
Gene expression levels in SubtiExpress: skfA | |
Interactions involving this protein in SubtInteract: SkfA | |
MW, pI | 5 kDa, 10.648 |
Gene length, protein length | 165 bp, 55 aa |
Immediate neighbours | ybcM, skfB |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
toxins, antitoxins and immunity against toxins, prophage 1
This gene is a member of the following regulons
AbrB regulon, PhoP regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU01910
Phenotypes of a mutant
Database entries
- BsubCyc: BSU01910
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: the mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine PubMed, the thioether bond is generated by SkfB PubMed
- Cofactor(s):
- Effectors of protein activity:
- Localization: secreted (according to Swiss-Prot)
Database entries
- BsubCyc: BSU01910
- Structure:
- UniProt: O31422
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript.
- The skfA transcript is stabilized by a stem-loop structure in the skfA-skfB intergenic region. PubMed
- The mRNA is very stable (half-life > 15 min) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- GP1645 ymdB::spc skf-gfp (spc) in the NCIB3610 background, available in Jörg Stülke's lab
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original Publications
Additional publications: PubMed