Difference between revisions of "SkfA"

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(Biological materials)
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* '''GFP fusion:'''
 
* '''GFP fusion:'''
 +
** GP1645 ''[[ymdB]]''::spc ''skf-gfp'' (spc) in the NCIB3610 background, available in [[ Jörg Stülke]]'s lab
 +
  
 
* '''two-hybrid system:'''  
 
* '''two-hybrid system:'''  

Revision as of 15:51, 3 February 2015

  • Description: spore killing factor

Gene name skfA
Synonyms ybcO
Essential no
Product spore killing factor
Function killing of sister cells
Gene expression levels in SubtiExpress: skfA
Interactions involving this protein in SubtInteract: SkfA
MW, pI 5 kDa, 10.648
Gene length, protein length 165 bp, 55 aa
Immediate neighbours ybcM, skfB
Sequences Protein DNA DNA_with_flanks
Genetic context
YbcO context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SkfA expression.png















Categories containing this gene/protein

toxins, antitoxins and immunity against toxins, prophage 1

This gene is a member of the following regulons

AbrB regulon, PhoP regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU01910

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: the mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine PubMed, the thioether bond is generated by SkfB PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed by glucose (33-fold) PubMed
    • expressed under conditions that trigger sporulation (Spo0A) PubMed
    • repressed during logrithmic growth (AbrB) PubMed
    • induced by phosphate starvation (PhoP) PubMed
    • induced by rhamnogalacturonan PubMed
  • Additional information:
    • Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript.
    • The skfA transcript is stabilized by a stem-loop structure in the skfA-skfB intergenic region. PubMed
    • The mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
    • GP1645 ymdB::spc skf-gfp (spc) in the NCIB3610 background, available in Jörg Stülke's lab


  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original Publications

Additional publications: PubMed