YocH

• Description: peptidoglycan hydrolase (amidase)
  
  

Gene name	yocH
Synonyms
Essential	no
Product	peptidoglycan hydrolase
Function	cell wall turnover
Gene expression levels in SubtiExpress: yocH
MW, pI	30 kDa, 8.371
Gene length, protein length	861 bp, 287 aa
Immediate neighbours	desR, yocI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

AbrB regulon, Spo0A regulon, WalR regulon

The gene

Basic information

• Locus tag: BSU19210

Database entries

• BsubCyc: BSU19210

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • contains two N-acetylglucosamine-polymer-binding LysM domains PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed

Database entries

• BsubCyc: BSU19210

• Structure:

• UniProt: O34669

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yocH PubMed

• Expression browser: yocH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during logrithmic growth (AbrB) PubMed
  • induced in response to cell wall derived muropeptides derived from growing cells but not lysed cells (requires activities of PrkC and YocH) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed
  • AbrB: transcription repression PubMed
  • WalR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • AH024 knock-out mutant (kan) available in the lab of Kevin Devine
  • BKE19210 (yocH::erm, available in the BGSC and in Jörg Stülke's lab)

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Kevin Devine