Difference between revisions of "RnhB"
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* '''Mutant:''' BP423 (''ΔrnhB::aphA3'') available in [[Fabian Commichau]]'s lab | * '''Mutant:''' BP423 (''ΔrnhB::aphA3'') available in [[Fabian Commichau]]'s lab | ||
| − | * '''Expression vector:''' | + | * '''Expression vector:''' ** for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [[SPINE]], expression from plasmid: pBP500 (E. coli amp & B. subtilis E/L) , available in [[Fabian Commichau]]'s lab |
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
| Line 133: | Line 133: | ||
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| − | * '''two-hybrid system:''' | + | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Fabian Commichau]]'s lab |
* '''Antibody:''' | * '''Antibody:''' | ||
Revision as of 13:23, 7 October 2014
- Description: RNase HII, endoribonuclease, responsible for removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication
| Gene name | rnhB |
| Synonyms | rnh |
| Essential | no |
| Product | Mn2+-dependent RNase HII |
| Function | endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules |
| Gene expression levels in SubtiExpress: rnhB | |
| MW, pI | 28 kDa, 5.518 |
| Gene length, protein length | 765 bp, 255 aa |
| Immediate neighbours | rbgA, ylqG |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16060
Phenotypes of a mutant
- increased mutation rate PubMed
- a rnhB rnhC double mutant grows poorly PubMed
- a rnhB mutS-mutL mutant is not viable PubMed
Database entries
- BsubCyc: BSU16060
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)
- removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication PubMed
- Protein family: RNase HII family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU16060
- Structure:
- UniProt: O31744
- KEGG entry: [2]
- E.C. number: 3.1.26.4.
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: BP423 (ΔrnhB::aphA3) available in Fabian Commichau's lab
- Expression vector: ** for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from plasmid: pBP500 (E. coli amp & B. subtilis E/L) , available in Fabian Commichau's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Karolina A Majorek, Stanislaw Dunin-Horkawicz, Kamil Steczkiewicz, Anna Muszewska, Marcin Nowotny, Krzysztof Ginalski, Janusz M Bujnicki
The RNase H-like superfamily: new members, comparative structural analysis and evolutionary classification.
Nucleic Acids Res: 2014, 42(7);4160-79
[PubMed:24464998]
[WorldCat.org]
[DOI]
(I p)
Takashi Tadokoro, Shigenori Kanaya
Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes.
FEBS J: 2009, 276(6);1482-93
[PubMed:19228197]
[WorldCat.org]
[DOI]
(I p)
Original publications
Nina Y Yao, Jeremy W Schroeder, Olga Yurieva, Lyle A Simmons, Mike E O'Donnell
Cost of rNTP/dNTP pool imbalance at the replication fork.
Proc Natl Acad Sci U S A: 2013, 110(32);12942-7
[PubMed:23882084]
[WorldCat.org]
[DOI]
(I p)
Sanae Fukushima, Mitsuhiro Itaya, Hiroaki Kato, Naotake Ogasawara, Hirofumi Yoshikawa
Reassessment of the in vivo functions of DNA polymerase I and RNase H in bacterial cell growth.
J Bacteriol: 2007, 189(23);8575-83
[PubMed:17905985]
[WorldCat.org]
[DOI]
(I p)
Mitsuru Haruki, Yasuo Tsunaka, Masaaki Morikawa, Shigenori Kanaya
Cleavage of a DNA-RNA-DNA/DNA chimeric substrate containing a single ribonucleotide at the DNA-RNA junction with prokaryotic RNases HII.
FEBS Lett: 2002, 531(2);204-8
[PubMed:12417313]
[WorldCat.org]
[DOI]
(P p)
M Itaya, A Omori, S Kanaya, R J Crouch, T Tanaka, K Kondo
Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168.
J Bacteriol: 1999, 181(7);2118-23
[PubMed:10094689]
[WorldCat.org]
[DOI]
(P p)
N Ohtani, M Haruki, M Morikawa, R J Crouch, M Itaya, S Kanaya
Identification of the genes encoding Mn2+-dependent RNase HII and Mg2+-dependent RNase HIII from Bacillus subtilis: classification of RNases H into three families.
Biochemistry: 1999, 38(2);605-18
[PubMed:9888800]
[WorldCat.org]
[DOI]
(P p)
