IolW

• Description: scyllo-inositol dehydrogenase, general stress protein
  
  

Gene name	yvaA
Synonyms
Essential	no
Product	scyllo-inositol dehydrogenase
Function	unknown
Gene expression levels in SubtiExpress: iolW
MW, pI	39 kDa, 5.457
Gene length, protein length	1074 bp, 358 aa
Immediate neighbours	csoR, azoR2
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU33530

Database entries

• BsubCyc: BSU33530

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: gfo/idh/mocA family (according to Swiss-Prot)

• Paralogous protein(s): YfiI, NtdC, YrbE, YteT, YulF, IolG

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU33530

• Structure: 3GFG

• UniProt: O32223

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: iolW PubMed

• Sigma factor: SigB PubMed

• Regulation:
  • induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 313 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1480 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 5476 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 3799 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 6429 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kosei Tanaka, Shintaro Tajima, Shinji Takenaka, Ken-ichi Yoshida
An improved Bacillus subtilis cell factory for producing scyllo-inositol, a promising therapeutic agent for Alzheimer's disease.
Microb Cell Fact: 2013, 12;124
[PubMed:24325193] [WorldCat.org] [DOI] (I e)

Tetsuro Morinaga, Hitoshi Ashida, Ken-Ichi Yoshida
Identification of two scyllo-inositol dehydrogenases in Bacillus subtilis.
Microbiology (Reading): 2010, 156(Pt 5);1538-1546
[PubMed:20133360] [WorldCat.org] [DOI] (I p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)