Difference between revisions of "MtnK"
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 5079 {{PubMed|24696501}} | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 5079 {{PubMed|24696501}} | ||
** number of protein molecules per cell (complex medium with amino acids, without glucose): 16333 {{PubMed|24696501}} | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 16333 {{PubMed|24696501}} | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 13437 {{PubMed|21395229}} | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 7764 {{PubMed|21395229}} | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 10257 {{PubMed|21395229}} | ||
=Biological materials = | =Biological materials = | ||
− | |||
* '''Mutant:''' | * '''Mutant:''' | ||
Revision as of 14:19, 17 April 2014
- Description: 5-methylthioribose kinase
Gene name | mtnK |
Synonyms | ykrT |
Essential | no |
Product | 5-methylthioribose kinase |
Function | methionine salvage |
Gene expression levels in SubtiExpress: mtnK | |
Metabolic function and regulation of this protein in SubtiPathways: mtnK | |
MW, pI | 45 kDa, 4.856 |
Gene length, protein length | 1197 bp, 399 aa |
Immediate neighbours | mtnA, mtnU |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids, membrane proteins, phosphoproteins, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13560
Phenotypes of a mutant
Database entries
- BsubCyc: BSU13560
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + S-methyl-5-thio-D-ribose = ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate (according to Swiss-Prot)
- Protein family: methylthioribose kinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- phosphorylated on Arg-80 OR Arg-82 and Arg-365 PubMed
- Effectors of protein activity:
- Localization:
- membrane associated PubMed
Database entries
- BsubCyc: BSU13560
- Structure: 2PUP
- UniProt: O31663
- KEGG entry: [3]
- E.C. number: 2.7.1.100
Additional information
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
- Additional information:
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
- belongs to the 100 most abundant proteins PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 5079 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 16333 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 13437 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 7764 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 10257 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References