Difference between revisions of "MtnK"

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** number of protein molecules per cell (minimal medium with glucose and ammonium): 5079 {{PubMed|24696501}}
 
** number of protein molecules per cell (minimal medium with glucose and ammonium): 5079 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 16333 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 16333 {{PubMed|24696501}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 13437 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 7764 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 10257 {{PubMed|21395229}}
  
 
=Biological materials =
 
=Biological materials =
 
 
* '''Mutant:'''
 
* '''Mutant:'''
  

Revision as of 14:19, 17 April 2014

  • Description: 5-methylthioribose kinase

Gene name mtnK
Synonyms ykrT
Essential no
Product 5-methylthioribose kinase
Function methionine salvage
Gene expression levels in SubtiExpress: mtnK
Metabolic function and regulation of this protein in SubtiPathways:
mtnK
MW, pI 45 kDa, 4.856
Gene length, protein length 1197 bp, 399 aa
Immediate neighbours mtnA, mtnU
Sequences Protein DNA DNA_with_flanks
Genetic context
YkrT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MtnK expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, membrane proteins, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

S-box

The gene

Basic information

  • Locus tag: BSU13560

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + S-methyl-5-thio-D-ribose = ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate (according to Swiss-Prot)
  • Protein family: methylthioribose kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • phosphorylated on Arg-80 OR Arg-82 and Arg-365 PubMed
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation: induced by methionine starvation (S-box) PubMed
  • Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
  • Additional information:
    • subject to Clp-dependent proteolysis upon glucose starvation PubMed
    • belongs to the 100 most abundant proteins PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 5079 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 16333 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 13437 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 7764 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 10257 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References