Difference between revisions of "RocD"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40340&redirect=T BSU40340] | ||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rocDEF.html] | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rocDEF.html] | ||
| Line 95: | Line 96: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40340&redirect=T BSU40340] | ||
* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3RUY 3RUY] (from ''B. anthracis'') | * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3RUY 3RUY] (from ''B. anthracis'') | ||
Revision as of 15:16, 2 April 2014
- Description: ornithine transaminase
| Gene name | rocD |
| Synonyms | |
| Essential | no |
| Product | ornithine transaminase |
| Function | arginine, ornithine and citrulline utilization |
| Gene expression levels in SubtiExpress: rocD | |
| Metabolic function and regulation of this protein in SubtiPathways: rocD | |
| MW, pI | 43 kDa, 4.894 |
| Gene length, protein length | 1203 bp, 401 aa |
| Immediate neighbours | rocE, rocR |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
AhrC regulon, CodY regulon, RocR regulon, SigL regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU40340
Phenotypes of a mutant
Database entries
- BsubCyc: BSU40340
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid (according to Swiss-Prot)
- Protein family: OAT subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU40340
- Structure: 3RUY (from B. anthracis)
- UniProt: P38021
- KEGG entry: [3]
- E.C. number: 2.6.1.13
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP656, aphA3, available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Adrienne Zaprasis, Tamara Hoffmann, Guido Wünsche, Lope A Flórez, Jörg Stülke, Erhard Bremer
Mutational activation of the RocR activator and of a cryptic rocDEF promoter bypass loss of the initial steps of proline biosynthesis in Bacillus subtilis.
Environ Microbiol: 2014, 16(3);701-17
[PubMed:23869754]
[WorldCat.org]
[DOI]
(I p)
Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647]
[WorldCat.org]
[DOI]
(P p)
Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455]
[WorldCat.org]
[DOI]
(P p)
R Gardan, G Rapoport, M Débarbouillé
Expression of the rocDEF operon involved in arginine catabolism in Bacillus subtilis.
J Mol Biol: 1995, 249(5);843-56
[PubMed:7540694]
[WorldCat.org]
[DOI]
(P p)
