MutS

• Description: DNA mismatch repair (mismatch recognition protein)
  
  

Gene name	mutS
Synonyms
Essential	no
Product	mismatch recognition protein
Function	DNA repair
Gene expression levels in SubtiExpress: mutS
Interactions involving this protein in SubtInteract: MutS
MW, pI	97 kDa, 5.189
Gene length, protein length	2574 bp, 858 aa
Immediate neighbours	cotE, mutL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU17040

Phenotypes of a mutant

• 60-fold increased mutation rate PubMed
• a rnhB mutS-mutL mutant is not viable PubMed

Database entries

• BsubCyc: BSU17040

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • MutS recognizes mismatches in the DNA and recruits MutL to the site of mismatch recognition

• Protein family: DNA mismatch repair mutS family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed
  • MutS-DnaN PubMed
  • DnaE-MutS PubMed
  • MutL-MutS PubMed

• Localization:
  • forms foci at midcell position, the frequency of foci increases upon mismatch formation PubMed
  • replication-active region of the DNA, via DnaN PubMed

Database entries

• BsubCyc: BSU17040

• Structure:
  • 1NG9 (from E. coli, 39% identity) PubMed

• UniProt: P49849

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mutS-mutL PubMed

• Expression browser: mutS PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • An antisense RNA is predicted for mutS PubMed
  • intracellular concentration: about 80 dimers (100 nM) PubMed

Biological materials

• Mutant:
  • GP1190 (del mutS-mutL::aphA3) PubMed, available in Jörg Stülke's lab
  • 1A833 ( mutS::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)