SspB

• Description: small acid-soluble spore protein (major beta-type SASP)
  
  

Gene name	sspB
Synonyms
Essential	no
Product	small acid-soluble spore protein (major beta-type SASP)
Function	protection of spore DNA
Gene expression levels in SubtiExpress: sspB
MW, pI	6 kDa, 4.791
Gene length, protein length	201 bp, 67 aa
Immediate neighbours	yheF, yheE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

• Locus tag: BSU09750

Phenotypes of a mutant

• a sspB mutant is sensitive to ionizing radiation PubMed

Database entries

• BsubCyc: BSU09750

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: alpha/beta-type SASP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU09750

• Structure:

• UniProt: P04832

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sspB PubMed

• Expression browser: sspB PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed during sporulation in the forespore (SigG, SpoVT) PubMed
  • the mRNA accumulates in a ymdB mutant PubMed

• Regulatory mechanism:
  • SpoVT: transcription activation PubMed

• Additional information:
  • the mRNA half-life is about 13 min PubMed

Biological materials

• Mutant:
  • 1S111 (no resistance), PubMed, available at BGSC
  • 1S113 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion: GP996 (cat, based on pSG1151), available in the Stülke lab

• two-hybrid system:

• Antibody: