SpoIIIJ

• Description: Sec-independent membrane protein translocase, essential for SigG activity at stage III
  
  

Gene name	spoIIIJ
Synonyms	spo0J87
Essential	no
Product	membrane protein translocase
Function	membrane insertion of proteins and protein secretion
Gene expression levels in SubtiExpress: spoIIIJ
Interactions involving this protein in SubtInteract: SpoIIIJ
MW, pI	29 kDa, 9.992
Gene length, protein length	783 bp, 261 aa
Immediate neighbours	jag, rnpA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein secretion, sporulation/ other, membrane proteins

The gene

Basic information

• Locus tag: BSU41040

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: membrane protein translocase, facilitates insertion of SpoIIIAE into the membrane PubMed

• Protein family: YidC/Oxa1/Alb3 family PubMed

• Paralogous protein(s): YidC2, one of the two proteins has to be present for viability PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • SpoIIIAE-SpoIIIJ PubMed
  • SpoIIIJ-MifM PubMed

• Localization:
  • membrane PubMed

Database entries

• Structure:

• UniProt: Q01625

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIIJ-jag PubMed

• Expression browser: spoIIIJ PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard University, Cambridge, USA homepage

Adriano Henriques, Lisbon, Portugal homepage

References

Reviews

Ross E Dalbey, Peng Wang, Jan Maarten van Dijl
Membrane proteases in the bacterial protein secretion and quality control pathway.
Microbiol Mol Biol Rev: 2012, 76(2);311-30
[PubMed:22688815] [WorldCat.org] [DOI] (I p)

Soledad Funes, Frank Kauff, Eli O van der Sluis, Martin Ott, Johannes M Herrmann
Evolution of YidC/Oxa1/Alb3 insertases: three independent gene duplications followed by functional specialization in bacteria, mitochondria and chloroplasts.
Biol Chem: 2011, 392(1-2);13-9
[PubMed:21194367] [WorldCat.org] [DOI] (I p)

Peng Wang, Ross E Dalbey
Inserting membrane proteins: the YidC/Oxa1/Alb3 machinery in bacteria, mitochondria, and chloroplasts.
Biochim Biophys Acta: 2011, 1808(3);866-75
[PubMed:20800571] [WorldCat.org] [DOI] (P p)

Martin van der Laan, Nico P Nouwen, Arnold J M Driessen
YidC--an evolutionary conserved device for the assembly of energy-transducing membrane protein complexes.
Curr Opin Microbiol: 2005, 8(2);182-7
[PubMed:15802250] [WorldCat.org] [DOI] (P p)

Original publications

Yanping Geng, Jeanine de Keyzer, Dirk-Jan Scheffers, Arnold J M Driessen
Defining the region of Bacillus subtilis SpoIIIJ that is essential for its sporulation-specific function.
J Bacteriol: 2014, 196(7);1318-24
[PubMed:24443530] [WorldCat.org] [DOI] (I p)

Chun-Kai Yang, Chung-Dar Lu, Phang C Tai
Differential expression of secretion machinery during bacterial growth: SecY and SecF decrease while SecA increases during transition from exponential phase to stationary phase.
Curr Microbiol: 2013, 67(6);682-7
[PubMed:23852076] [WorldCat.org] [DOI] (I p)

Shinobu Chiba, Koreaki Ito
Multisite ribosomal stalling: a unique mode of regulatory nascent chain action revealed for MifM.
Mol Cell: 2012, 47(6);863-72
[PubMed:22864117] [WorldCat.org] [DOI] (I p)

Shinobu Chiba, Anne Lamsa, Kit Pogliano
A ribosome-nascent chain sensor of membrane protein biogenesis in Bacillus subtilis.
EMBO J: 2009, 28(22);3461-75
[PubMed:19779460] [WorldCat.org] [DOI] (I p)

Manfred J Saller, Fabrizia Fusetti, Arnold J M Driessen
Bacillus subtilis SpoIIIJ and YqjG function in membrane protein biogenesis.
J Bacteriol: 2009, 191(21);6749-57
[PubMed:19717609] [WorldCat.org] [DOI] (I p)

Mónica Serrano, Filipe Vieira, Charles P Moran, Adriano O Henriques
Processing of a membrane protein required for cell-to-cell signaling during endospore formation in Bacillus subtilis.
J Bacteriol: 2008, 190(23);7786-96
[PubMed:18820020] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Amy H Camp, Richard Losick
A novel pathway of intercellular signalling in Bacillus subtilis involves a protein with similarity to a component of type III secretion channels.
Mol Microbiol: 2008, 69(2);402-17
[PubMed:18485064] [WorldCat.org] [DOI] (I p)

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)

Aileen Rubio, Xin Jiang, Kit Pogliano
Localization of translocation complex components in Bacillus subtilis: enrichment of the signal recognition particle receptor at early sporulation septa.
J Bacteriol: 2005, 187(14);5000-2
[PubMed:15995216] [WorldCat.org] [DOI] (P p)

Mónica Serrano, Luísa Côrte, Jason Opdyke, Charles P Moran, Adriano O Henriques
Expression of spoIIIJ in the prespore is sufficient for activation of sigma G and for sporulation in Bacillus subtilis.
J Bacteriol: 2003, 185(13);3905-17
[PubMed:12813085] [WorldCat.org] [DOI] (P p)

Harold Tjalsma, Sierd Bron, Jan Maarten van Dijl
Complementary impact of paralogous Oxa1-like proteins of Bacillus subtilis on post-translocational stages in protein secretion.
J Biol Chem: 2003, 278(18);15622-32
[PubMed:12586834] [WorldCat.org] [DOI] (P p)

Takako Murakami, Koki Haga, Michio Takeuchi, Tsutomu Sato
Analysis of the Bacillus subtilis spoIIIJ gene and its Paralogue gene, yqjG.
J Bacteriol: 2002, 184(7);1998-2004
[PubMed:11889108] [WorldCat.org] [DOI] (P p)

J Errington, L Appleby, R A Daniel, H Goodfellow, S R Partridge, M D Yudkin
Structure and function of the spoIIIJ gene of Bacillus subtilis: a vegetatively expressed gene that is essential for sigma G activity at an intermediate stage of sporulation.
J Gen Microbiol: 1992, 138(12);2609-18
[PubMed:1487728] [WorldCat.org] [DOI] (P p)