DctB

• Description: C4-dicarboxylate binding protein, co-sensor for DctS
  
  

Gene name	dctB
Synonyms	ydbE
Essential	no
Product	C4-dicarboxylate binding protein
Function	sensing of C4-dicarboxylates
Gene expression levels in SubtiExpress: dctB
Interactions involving this protein in SubtInteract: DctB
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	39 kDa, 4.889
Gene length, protein length	1050 bp, 350 aa
Immediate neighbours	ydbD, dctS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

The gene

Basic information

• Locus tag: BSU04440

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • not involved in C4-dicarboxylate transport PubMed
  • acts as co-sensor for DctS PubMed

• Protein family: bacterial solute-binding protein 7 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • DctB-DctS-DctP, sensing complex PubMed

• Localization:
  • secreted (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P96600

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dctB-ydbD PubMed

• Expression browser: dctB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sabrina Graf, Dominik Schmieden, Karolin Tschauner, Sabine Hunke, Gottfried Unden
The sensor kinase DctS forms a tripartite sensor unit with DctB and DctA for sensing C4-dicarboxylates in Bacillus subtilis.
J Bacteriol: 2014, 196(5);1084-93
[PubMed:24375102] [WorldCat.org] [DOI] (I p)

Kei Asai, Sang-Hoon Baik, Yasuhiro Kasahara, Shigeki Moriya, Naotake Ogasawara
Regulation of the transport system for C4-dicarboxylic acids in Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 2);263-271
[PubMed:10708364] [WorldCat.org] [DOI] (P p)