CwlS

• Description: D,L-endopeptidase, peptidoglycan hydrolase for cell separation
  
  

Gene name	cwlS
Synonyms	yojL
Essential	no
Product	D,L-endopeptidase, peptidoglycan hydrolase
Function	cell separation
Gene expression levels in SubtiExpress: cwlS
MW, pI	44 kDa, 10.438
Gene length, protein length	1242 bp, 414 aa
Immediate neighbours	yojM, yojK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, membrane proteins

This gene is a member of the following regulons

Abh regulon, AbrB regulon, CcpA regulon, SigD regulon, SigH regulon,

The gene

Basic information

• Locus tag: BSU19410

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of gamma-D-glutamyl bonds to the L-terminus (position 7) of meso-diaminopimelic acid (meso-A₂pm) in 7-(L-Ala-gamma-D-Glu)-meso-A₂pm and 7-(L-Ala-gamma-D-Glu)-7-(D-Ala)-meso-A₂pm (according to Swiss-Prot)

• Protein family: Cu-Zn superoxide dismutase family (according to Swiss-Prot)

• Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity

Extended information on the protein

• Kinetic information:

• Domains:
  • contains four N-acetylglucosamine-polymer-binding LysM domains PubMed
  • C-terminal D,L-endopeptidase domain PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)
  • localizes to cell septa and poles PubMed

Database entries

• Structure:

• UniProt: O31852

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: cwlS PubMed

• Sigma factor: SigD, SigH, according to PubMed

• Regulation:
  • repressed by glucose (3-fold) (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression
  • Abh and AbrB: synergistic transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Girbe Buist, Anton Steen, Jan Kok, Oscar P Kuipers
LysM, a widely distributed protein motif for binding to (peptido)glycans.
Mol Microbiol: 2008, 68(4);838-47
[PubMed:18430080] [WorldCat.org] [DOI] (I p)