YkoV

• Description: DNA-end-binding protein Ku, recruits LigD to DNA ends, confers dry-heat resistance to dormant spores PubMed

Gene name	ykoV
Synonyms
Essential	no
Product	DNA-end-binding protein Ku
Function	non-homologous end joining DNA repair, repair of gapped DNA substrates
Gene expression levels in SubtiExpress: ykoV
Interactions involving this protein in SubtInteract: YkoV
MW, pI	34 kDa, 7.213
Gene length, protein length	933 bp, 311 aa
Immediate neighbours	ligD, dgcW
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

• Locus tag: BSU13410

Phenotypes of a mutant

• sensitivity to ionizing radiation in the stationary phase PubMed
• sensitivity of spores to several DNA-damaging treatments known to cause double strand breaks, such as UV-ray, X-ray, ultrahigh vacuum and wet heat PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YkoV-LigD PubMed

• Localization:
  • associates with the nucleoid during germination PubMed

Database entries

• Structure:

• UniProt: O34859

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ykoV-ligD PubMed

• Expression browser: ykoV PubMed

• Sigma factor: SigG PubMed

• Regulation: expressed during sporulation in the forespore (SigG, SpoVT) PubMed

• Regulatory mechanism:
  • SpoVT: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • BP141 (ykoV-ligD::kan) available in Fabian Commichau's lab
  • BP142 (ykoW-ykoV-ligD::kan) available in Fabian Commichau's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Stewart Shuman, Michael S Glickman
Bacterial DNA repair by non-homologous end joining.
Nat Rev Microbiol: 2007, 5(11);852-61
[PubMed:17938628] [WorldCat.org] [DOI] (I p)

A J Doherty, S P Jackson
DNA repair: how Ku makes ends meet.
Curr Biol: 2001, 11(22);R920-4
[PubMed:11719239] [WorldCat.org] [DOI] (P p)

J M Jones, M Gellert, W Yang
A Ku bridge over broken DNA.
Structure: 2001, 9(10);881-4
[PubMed:11591342] [WorldCat.org] [DOI] (P p)

Original publications

Miguel de Vega
The minimal Bacillus subtilis nonhomologous end joining repair machinery.
PLoS One: 2013, 8(5);e64232
[PubMed:23691176] [WorldCat.org] [DOI] (I e)

Ralf Moeller, Erko Stackebrandt, Günther Reitz, Thomas Berger, Petra Rettberg, Aidan J Doherty, Gerda Horneck, Wayne L Nicholson
Role of DNA repair by nonhomologous-end joining in Bacillus subtilis spore resistance to extreme dryness, mono- and polychromatic UV, and ionizing radiation.
J Bacteriol: 2007, 189(8);3306-11
[PubMed:17293412] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Geoffrey R Weller, Boris Kysela, Rajat Roy, Louise M Tonkin, Elizabeth Scanlan, Marina Della, Susanne Krogh Devine, Jonathan P Day, Adam Wilkinson, Fabrizio d'Adda di Fagagna, Kevin M Devine, Richard P Bowater, Penny A Jeggo, Stephen P Jackson, Aidan J Doherty
Identification of a DNA nonhomologous end-joining complex in bacteria.
Science: 2002, 297(5587);1686-9
[PubMed:12215643] [WorldCat.org] [DOI] (I p)

L Aravind, E V Koonin
Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair system.
Genome Res: 2001, 11(8);1365-74
[PubMed:11483577] [WorldCat.org] [DOI] (P p)