GbsA

• Description: glycine betaine-aldehyde dehydrogenase, glycine betaine synthesis
  
  

Gene name	gbsA
Synonyms
Essential	no
Product	glycine betaine-aldehyde dehydrogenase
Function	osmoprotection
Gene expression levels in SubtiExpress: gbsA
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	53 kDa, 4.713
Gene length, protein length	1470 bp, 490 aa
Immediate neighbours	gbsB, gbsR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, coping with hyper-osmotic stress

This gene is a member of the following regulons

GbsR regulon

The gene

Basic information

• Locus tag: BSU31060

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Betaine aldehyde + NAD⁺ + H₂O = betaine + NADH (according to Swiss-Prot)

• Protein family: aldehyde dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s): YfmT, AldY, YcbD, GabD, DhaS, IolA, PutC, RocA, AldX, YwdH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3RHH (from from Bacillus halodurans C-125 complexed with NADP, 31% identity, 67% similarity)

• UniProt: P71016

• KEGG entry: [2]

• E.C. number: 1.2.1.8

Expression and regulation

• Operon: gbsA-gbsB PubMed

• Expression browser: gbsA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by choline (GbsR) PubMed

• Regulatory mechanism:
  • GbsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A1056 ( gbsA::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Erhard Bremer, University of Marburg, Germany homepage

References

Gabriele Nau-Wagner, Daniela Opper, Anne Rolbetzki, Jens Boch, Bettina Kempf, Tamara Hoffmann, Erhard Bremer
Genetic control of osmoadaptive glycine betaine synthesis in Bacillus subtilis through the choline-sensing and glycine betaine-responsive GbsR repressor.
J Bacteriol: 2012, 194(10);2703-14
[PubMed:22408163] [WorldCat.org] [DOI] (I p)

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)

J Boch, G Nau-Wagner, S Kneip, E Bremer
Glycine betaine aldehyde dehydrogenase from Bacillus subtilis: characterization of an enzyme required for the synthesis of the osmoprotectant glycine betaine.
Arch Microbiol: 1997, 168(4);282-9
[PubMed:9297465] [WorldCat.org] [DOI] (P p)

J Boch, B Kempf, R Schmid, E Bremer
Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes.
J Bacteriol: 1996, 178(17);5121-9
[PubMed:8752328] [WorldCat.org] [DOI] (P p)