SigW

• Description: RNA polymerase ECF-type sigma factor SigW, activated by alkaline shock and by polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100
  
  

Gene name	sigW
Synonyms	ybbL
Essential	no
Product	RNA polymerase ECF-type sigma factor SigW
Function	resistance against SdpC that functions in detoxification and/or production of antimicrobial compounds
Gene expression levels in SubtiExpress: sigW
Interactions involving this protein in SubtInteract: SigW
MW, pI	21 kDa, 8.614
Gene length, protein length	561 bp, 187 aa
Immediate neighbours	trnSL-Gln2, rsiW
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

AbrB regulon, SigW regulon

The gene

Basic information

• Locus tag: BSU01730

Phenotypes of a mutant

• sensitive to ß-lactam antibiotics such as cefuroxime and to fosfomycin PubMed
• Inactivation of alsR, sigD and sigW increases competitive fitness of Bacillus subtilis under non-sporulating conditions PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ECF subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: RsiW acts as antagonist of SigW (anti-SigW)

• Interactions:
  • RsiW-SigW PubMed
  • SigW-RpoB-RpoC

• Localization:

Database entries

• Structure:

• UniProt: Q45585

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sigW-rsiW PubMed

• Expression browser: sigW PubMed

• Sigma factor: SigW PubMed

• Regulation:
  • induced under conditions of alkali shock or in the presence of the toxin SdpC (SigW) PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A905 ( sigW::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Thomas Wiegert, University of Bayreuth, Germany Homepage

References

Reviews

Theresa D Ho, Craig D Ellermeier
Extra cytoplasmic function σ factor activation.
Curr Opin Microbiol: 2012, 15(2);182-8
[PubMed:22381678] [WorldCat.org] [DOI] (I p)