TrxA

• Description: antioxidative action by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange
  
  

Gene name	trxA
Synonyms	trx
Essential	yes PubMed
Product	thioredoxin
Function	protection of proteins against oxidative damage
Gene expression levels in SubtiExpress: trxA
Interactions involving this protein in SubtInteract: TrxA
MW, pI	11 kDa, 4.308
Gene length, protein length	312 bp, 104 aa
Immediate neighbours	uvrC, abf2
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other, general stress proteins (controlled by SigB), resistance against oxidative and electrophile stress, essential genes

This gene is a member of the following regulons

CtsR regulon, SigB regulon, Spx regulon

The gene

Basic information

• Locus tag: BSU28500

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: thioredoxin family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ArsC-TrxA PubMed

• Localization: cytoplasm PubMed

Database entries

• Structure: 2GZZ (oxidized form), 2GZY (reduced form), 2IPA (TrxA-ArsC-complex) PubMed

• UniProt: P14949

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: trxA PubMed

• Expression browser: trxA PubMed

• Sigma factor: SigA PubMed, SigB PubMed

• Regulation:
  • induced by stress (SigB) PubMed
  • regulation by Spx in response to diamide stress
  • induced by heat stress (CtsR) PubMed

• Regulatory mechanism:
  • CtsR: transcription repression PubMed

• Additional information:
  • A ncRNA is predicted between trxA and abf2 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Jun Lu, Arne Holmgren
The thioredoxin antioxidant system.
Free Radic Biol Med: 2014, 66;75-87
[PubMed:23899494] [WorldCat.org] [DOI] (I p)