Difference between revisions of "Eno"

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* '''Mutant:'''  
 
* '''Mutant:'''  
** GP594 (''eno''::''cat''), available in [[Jörg Stülke]]'s lab  
+
** GP594 (''eno''::''cat''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}
** GP599 (''eno''::''erm''), available in [[Jörg Stülke]]'s lab  
+
** GP599 (''eno''::''erm''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}
** GP698 (''eno''-''[[pgm]]''::''cat''), available in [[Jörg Stülke]]'s lab
+
** GP698 (''eno''-''[[pgm]]''::''cat''), available in [[Jörg Stülke]]'s lab, {{PubMed|23420519}}
  
 
* '''Expression vector:'''  
 
* '''Expression vector:'''  
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<pubmed> 8994873 </pubmed>
 
<pubmed> 8994873 </pubmed>
 
==Subcellular localization of enolase==
 
==Subcellular localization of enolase==
'''Additional publications:''' {{PubMed|15003462,21856851}}
+
<pubmed> 16479537 18763711 20497499 15003462,21856851</pubmed>
<pubmed> 16479537 18763711 20497499 </pubmed>
 
  
 
==Other original publications==
 
==Other original publications==

Revision as of 15:59, 13 July 2013

Gene name eno
Synonyms
Essential Yes (PubMed)
Product enolase
Function enzyme in glycolysis/ gluconeogenesis
Gene expression levels in SubtiExpress: eno
Interactions involving this protein in SubtInteract: Eno
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 46,4 kDa, 4.49
Gene length, protein length 1290 bp, 430 amino acids
Immediate neighbours yvbK, pgm
Sequences Protein DNA DNA_with_flanks
Genetic context
Eno context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Eno expression.png
















Categories containing this gene/protein

carbon core metabolism, essential genes, membrane proteins, phosphoproteins, universally conserved proteins

This gene is a member of the following regulons

CggR regulon

The gene

Basic information

  • Locus tag: BSU33900

Phenotypes of a mutant

  • no growth on LB, requires glucose and malate
  • essential according to Kobayashi et al. on LB PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O (according to Swiss-Prot) 2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
  • Protein family: enolase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: reversible Michaelis-Menten PubMed
  • Domains:
    • substrate binding domain (366–369)
  • Modification: phosphorylation on Thr-141 AND Ser-259 AND Tyr-281 AND Ser-325 PubMed, PubMed, PubMed
  • Cofactor(s): Mg2+
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism: transcription repression by CggR PubMed
  • Additional information:

Biological materials

  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany Homepage

Your additional remarks

References

Reviews

G H Reed, R R Poyner, T M Larsen, J E Wedekind, I Rayment
Structural and mechanistic studies of enolase.
Curr Opin Struct Biol: 1996, 6(6);736-43
[PubMed:8994873] [WorldCat.org] [DOI] (P p)

Subcellular localization of enolase


Other original publications