YcbD

• Description: NADP(+)-dependent alpha-ketoglutaric semialdehyde dehydrogenase
  
  

Gene name	ycbD
Synonyms
Essential	no
Product	NADP(+)-dependent alpha-ketoglutaric semialdehyde dehydrogenase
Function	utilization of D-glucarate/galactarate
Gene expression levels in SubtiExpress: ycbD
MW, pI	52 kDa, 4.787
Gene length, protein length	1464 bp, 488 aa
Immediate neighbours	ycbC, ycbE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

YcbG regulon

The gene

Basic information

• Locus tag: BSU02470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: an aldehyde + NAD⁺ + H₂O = an acid + NADH (according to Swiss-Prot)

• Protein family: aldehyde dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s): YfmT, AldY, GabD, GbsA, DhaS, IolA, PutC, RocA, AldX, YwdH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3RHH (from from Bacillus halodurans C-125 complexed with NADP, 36% identity, 69% similarity)

• UniProt: P42236

• KEGG entry: [3]

• E.C. number: 1.2.1.3

Expression and regulation

• Operon ycbC-ycbD-ycbE-ycbF-ycbG-ycbH-ycbJ PubMed

• Expression browser: ycbD PubMed

• Sigma factor:

• Regulation: induced by D-glucarate/galactarate (YcbG) PubMed

• Regulatory mechanism: YcbG: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Seiya Watanabe, Masaki Yamada, Iwao Ohtsu, Keisuke Makino
alpha-ketoglutaric semialdehyde dehydrogenase isozymes involved in metabolic pathways of D-glucarate, D-galactarate, and hydroxy-L-proline. Molecular and metabolic convergent evolution.
J Biol Chem: 2007, 282(9);6685-95
[PubMed:17202142] [WorldCat.org] [DOI] (P p)

Shigeo Hosoya, Kunio Yamane, Michio Takeuchi, Tsutomu Sato
Identification and characterization of the Bacillus subtilis D-glucarate/galactarate utilization operon ycbCDEFGHJ.
FEMS Microbiol Lett: 2002, 210(2);193-9
[PubMed:12044674] [WorldCat.org] [DOI] (P p)