Difference between revisions of "SinI"

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|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[biofilm formation]]
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[biofilm formation]]
 
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU24600 sinI]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24600 sinI]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SinI SinI]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SinI SinI]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqhG]]'', ''[[sinR]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqhG]]'', ''[[sinR]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14391&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24600 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24600 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24600 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:sinI_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:sinI_context.gif]]

Revision as of 13:17, 13 May 2013

  • Description: antagonist of SinR, drives SlrR from the SlrR(LOW) to the SlrR(HIGH) state

Gene name sinI
Synonyms
Essential no
Product antagonist of SinR
Function control of biofilm formation
Gene expression levels in SubtiExpress: sinI
Interactions involving this protein in SubtInteract: SinI
Regulation of this protein in SubtiPathways:
Biofilm
MW, pI 6 kDa, 6.333
Gene length, protein length 171 bp, 57 aa
Immediate neighbours yqhG, sinR
Sequences Protein DNA Advanced_DNA
Genetic context
SinI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SinI expression.png















Categories containing this gene/protein

transcription factors and their control, transition state regulators, biofilm formation

This gene is a member of the following regulons

AbrB regulon, ScoC regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU24600

Phenotypes of a mutant

  • altered cell death pattern in colonies PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): SlrA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • activated under conditions that trigger sporulation by (Spo0A) interaction PubMed,PubMed
    • repressed during exponential growth (ScoC) PubMed
    • repressed during logrithmic growth (AbrB) PubMed
    • repressed during vegetative growth (SinR) PubMed
  • Additional information:
    • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

  • Mutant:
    • GP959 (spc), available in Stülke lab
    • DS91 (spc) NCIB3610 derivate, available in Stülke lab
    • 1S98 ( sinI::kan), PubMed, available at BGSC
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • FLAG-tag construct (C-term): GP935 (kan), available in Stülke lab
  • Antibody:

Labs working on this gene/ protein

Your additional remarks

References

Reviews

Patrick Piggot
Epigenetic switching: bacteria hedge bets about staying or moving.
Curr Biol: 2010, 20(11);R480-2
[PubMed:20541494] [WorldCat.org] [DOI] (I p)

Modelling of the SinI/SinR switch

Jennifer S Hallinan, Goksel Misirli, Anil Wipat
Evolutionary computation for the design of a stochastic switch for synthetic genetic circuits.
Annu Int Conf IEEE Eng Med Biol Soc: 2010, 2010;768-74
[PubMed:21095906] [WorldCat.org] [DOI] (P p)

Original publications

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